For removal of micro contamination from the explants many sterilizing chemicals have been used. The disinfectants have many advantages as well as disadvantages. The effects of three commonly used sterilants i.e. mercuric chloride, sodium hypochlorite and calcium hypochlorite on explants treated at different concentration and exposure time are studied in the present experiment. Result showed that the bacterial contamination was reduced to 10 % in mercuric chloride (S8), 20 % in sodium hypochlorite (S16) and 15 % in calcium hypochlorite (S22) where as the fungal contamination was reduced to 5 % in all the cases. But the percentage of explants survived decreases with increase in concentration and exposure time due to the toxic effects of the sterilant.