Glutathione reductase (GR) is an essential enzyme which plays an important role in the Ascorbate Glutathione metabolic pathway for the oxidative tolerance in plants. It acts as an anti-oxidant enzyme preserving a reduced intracellular environment and protection of cellular macromolecules like DNA, proteins and lipids. During exposure to the biotic stresses, the differential modulation of GR in plants has been widely implicated in the significance of antioxidants as the major components of plant defense operations. How the stress factor affects the plant leaves and what is the role of GR as a defense mechanism to protect the plants from these stresses, will be studied in this review in a gross detail. This review lays emphasis on the overview about structure, localization, biosynthesis and mechanism of GR from the abiotic stress for exposed crop plants and also points-out unexplored aspects in the current context. It draws inspiration from a number of observations and research papers by scientists across the molecular biology world to understand and assert that GR is a vital enzyme without which a plant’s survival is difficult and impossible. We hope that this review would enlighten the reader to think broadly about the plant’s life cycle and importance of GR as a performance enhancing-agent in plants to help maintain the balance between plant and animals in the environment.