ISOLATION AND DEGRADING OF ENGINE OIL BY PSEUDOMONAS AERUGINOSA
Vinothini. C, Sudhakaran.S and Ravikumar.R
Abstract
The soil sample was collected at the field near truck workshop at Namakkal district that was disposed with used motor oil discharged from truck and uncontaminated soil sample also collected in same field. The pH of the spent engine oil contaminated soil is slightly acidic when compared to that of the uncontaminated soil. The spent engine oil contaminated soil had a higher conductivity, total organic carbon and phosphorus when compared to the uncontaminated soil. The spent engine oil degradation bacteria was isolated and identified as Bacillus sp.HC-1 and HC-3. and Pseudomonas sp. HC-2 and HC-4. Among the four isolates, Pseudomonas sp. HC-2 exhibited the maximum degradation of spent oil. Pseudomonas and Bacillus isolates were degraded upto 1 and 2 % concentration respectively. The 3 % and 4 % inoculum size was optimum for Pseudomonas sp. and Bacillus sp. respectively. The degradation was increased with increase the incubation periods. Among the 4 isolates, HC-2 was efficient for degradation of spent engine oil. Based on 16SrRNA technique, HC-2 strain was designated as Pseudomonas aeruginosa HC-2 which involved in spent oil degradation.