PRODUCTION OF LACCASE ENZYME BY WHITE ROT FUNGI CORIOLUS VERSICOLOR
Anju Rani, Vineet Girdharwal, Raj Singh, Amit Kumar and Gyanika. Shukla
White rot fungi such as Coriolus versicolor is known producer of lignolytic enzymes that are involved in the natural delignification of wood. Thirteen fungal samples were isolated from soil samples by serial dilution of 1 gm soil in 10 mL water. Dilutions of sample (10-5and 10-6) were prepared and transferred 0.1 mL of each dilution into malt extract agar medium plates supplemented with streptomycin and incubated at 350C for 3-5 days and named as F2-F14. Out of 13 samples one strain F3 from soil samples compared with F1 from rotted wood was selected for further studies. After three days selected fungal strains for ligninolytic activity were observed for the appearance of brown, yellow and green colours respectively. Ligninolytic activities of the selected fungal strains were observed positive in F2¬, F3, F5, F6, F7 and F14, whereas culture no. F4 and F10 showed negative results. The maximum and minimum diameter of fungal colony was recorded as 9.4 cm and 2.3 cm respectively, in F1 strain. The study revealed that maximum laccase activity was observed after 16 days of incubation period in CuSO4 whereas in veratryl alcohol that was recorded after 10 days of incubation periods, whereas minimum laccase activity was recorded in CuSO4 after 4 days of incubation period whereas in veratryl alcohol that was recorded after 16 day of incubation period.
Keywords: Coriolus versicolor, laccase enzyme, ligninolytic enzymes, malt extract agar medium, CuSO4 and veratrly alcohol.