The present study was conducted to determine the effect of different doses of six chemical mutagens i.e. ethyl methane sulphonate (EMS), Colchicine, PEG, Sodium Azide, 2,4-D and Acridine Orange on seed germination and callus induction in Dendrocalamus hamiltonii. The aim of this study was to identify the effects of different percentages of mutagen on the seed germination, root and shoot growth and in vitro callus induction for determining most effective dose for successful mutagenesis research studies. For this purpose, the seeds of Dendrocalamus hamiltonii were treated with 0.001, 0.01, 0.1, 0.2, and 0.3% EMS, Colchicine, PEG, Sodium Azide, 2,4-D and Acridine Orange doses. Germination percentage of seed, survival percentage of seedling, callus induction from seeds and callus growth were evaluated in comparison to the untreated materials. Effect of mutagen treatments showed statistically significant differences among the evaluated characters. The seed germination percentage decreased with increase in the concentration/doses when compared to control. The percent callus induction delayed with the increase in the concentration of mutagen. Maximum percent germination (92 %) was observed in control followed by 0.001% PEG (53.3%), 0.001% 2,4-D ( and 0.01% Acridine orange (50%).